RE: [Histonet] Understained my slides with hematoxylin

and the differentiation solution (after the haematoxylin - if you use it). Check the sections after blueing. If it looks too weak, increase the Hx staining time /or decrease the differentiation step. Counterstain with eosin as usual and you should be right. Regards Tony Henwood JP, MSc, BAppSc

[Histonet] RE: (reply) silly questions.---PFA

visited, as well as several Pathology Colleges (eg CAP and RCPA) surveys indicate that MOST (if not all) histopathology labs prepare their 10% formalin fixative from concentrated formalin not polyformaldehyde as you have incorrectly stated. Be careful of misinformation. Regards Tony Henwood JP

RE: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS

seconds. 9. Counterstain in Metanil Yellow solution, 6 seconds. 10. Rinse in distilled water, 5 seconds. 11. Blot, dehydrate rapidly, clear and mount. Results: Fungi stained purple to red against a yellow background. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] (reply) silly questions.---PFA

it is greater than 0.5% formalin Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

RE: [Histonet] at a troubleshooting loss

?carbon from lead pensils used for labelling slides. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag

RE: [Histonet] Silly Question?

Where is the evidence - or is this another mythical beast some of us believe? I don't believe it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road

RE: [Histonet] Silly Question?

lab, they follow my rules or take a different road. But having said this I will negotiate, hoping to get the best quality that we can. Communication (and sometimes education) is the key Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612

RE: [Histonet] Silly Question? - Need help quickly!

Good point Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

RE: [Histonet] PFA preparation

My experience is that when you add paraformaldehyde to water all it forms is a colloidal solution (ie on standing, the paraformaldehyde settles with very little going into solution (personal experience, waited one week, then gave up). Has your experience been different? Regards Tony Henwood

RE: [Histonet] IHC on fresh frozen

What? Do you roll it in the paraformaldehyde powder? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag

RE: RE: [Histonet] IHC on paraformaldehyde-fixed

tf wrote: I DO believe that one reason some people use 4% PFA rather 10% formalin is that PFA is a bit more stable, both for storage and transportation~~~. I have not heard this before. Do you have a reference for this? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

RE: [Histonet] IHC on paraformaldehyde-fixed

from paraformaldehyde. If a concentrated formalin solution (40% formaldehyde) is used, then it should be termed 10% formalin. If you do a search on Histonet for paraformaldehye, you will find that this topic has been extensively discussed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT

RE: [Histonet] as Thanksgiving approaches,what do you think abouta gall bladder in vinegar ?

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message

RE: [Histonet] Re: Telly's fixative

NINE years ago. Wow time flys, also God Bless You and check the spelling Tellnoniczky or Tellyesnicxky, Oh just give me a beer or Histoclear on the rocks! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

RE: [Histonet] RE: Uneven HE Staining

of placenta, has excess blood been removed from the placenta? Or have you checked the concentration of formalin (see Jaspers (1987) J Histotechnol 10 (4): 263-265 for the method using Schiffs reagent). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior

RE: [Histonet] Re: bone marrow biopsies

Only if he is well fixed first!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145

RE: [Histonet] Presidential Voting Infomation

From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street

RE: [Histonet] restarting DAB reaction

If we don't give it a go, how would we know? Now I know!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead

RE: [Histonet] restarting DAB reaction

Yes, I would also agree that this would probably be the case. I have not tried to redo the DAB step after counterstaining, dehydrating, clearing and mounting. I would expect it not to work. Though I could easily be corrected. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC

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