Hi, I've been trying to do hair follicle analysis in mouse skin sections for the past couple of months.I usually fix the skin in Neutral Buffer Formaline (NBF) for 24-48 hours and then send it to our histology department to be embedded in paraffin blocks. However,i'm repeatedly getting cross sections and broken hair follicles in my skin sections.Ive tried embedding skin supported on paper and cutting the blocks at various angles but it didn't make too much of a difference.If anything the stupid paper is ruining the cutting blades! Ive also tried cryosectioning snap frozen skin samples but that isn't working out very well either.Does anyone have any clue at all why this is happening and what i could possibly do to improve the section quality?
Thanks Vagisha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet