Re: [Histonet] Safranin O staining

Hi Liz,   Get it.  Thank you for the detailed procedures.   Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 20, 2013 12:15 AM Subject: RE: [Histonet] Safranin O staining Victor  

Re: [Histonet] Safranin O staining

processing.   BTW, I don't have access right to the paper. Anyway, the staining pictures on the website are very nice.    Once, thank you for your help.   Best Regards, Victor From: Elizabeth Chlipala To: Victor Wong ; Tony Henwood (SCHN) ; "histonet@lists.utsouthwestern.edu"

Re: [Histonet] Safranin O staining

r sources.   P.S. unforuntuately we don't have a Haematology department but may be we can try other cells.   Best Regards, Victor   From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 19, 2013 6:44 AM Subject: RE:

Re: [Histonet] Safranin O staining

arose may be deletrious to the staining.  As suggested, I will extend the fixation time.   Best Regards, Victor     From: "koelli...@comcast.net" To: Victor Wong Cc: histonet@lists.utsouthwestern.edu Sent: Monday, February 18, 2013 10:42 PM Subject: Re: [Histonet] Safranin O stain

Re: [Histonet] Safranin O staining

imes, or follow a known protocol (you can find that in either Peter Gray's or Lillie's books). René J. From: Victor Wong To: "histonet@lists.utsouthwestern.edu" Sent: Sunday, February 17, 2013 9:34 PM Subject: [Histonet] Safranin O staining Hi all, I am working on ind

Re: [Histonet] Safranin O staining

by chondrogenic pellet can be destroyed by heat?  Best Regards, Victor From: Tony Henwood (SCHN) To: 'Victor Wong' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 18, 2013 11:45 AM Subject: RE: [Histonet] Safranin O staining Victor, Heat can affect the way ti

Re: [Histonet] Safranin O staining

your processing protocol?   Best Regards, Victor   From: Tony Reilly To: "histonet@lists.utsouthwestern.edu" ; Victor Wong Sent: Monday, February 18, 2013 1:50 PM Subject: Re: [Histonet] Safranin O staining Hi Victor I have been using agar cell blocks for over 30 years.  It h

[Histonet] Safranin O staining

Hi all, I am working on induced chondrogenesis in cell culture.  After, induction, I put the clump of cells (pellets) in 2% agarose to make a cell block.  When the pellet did not sink to the bottom of agarose, I heated to melt the agarose again and centrifuged with higher speed. I fix the agar

[Histonet] PECAM-1 (sc-1506R) staining

Dear all,   Thank you for all the professional suggestion of the previous IHC staining and finally I got some positive results.   Then comes problem on the PECAM-1 IHC.  I am using the Santa Cruz sc-1506R (it is a anti-RAT antibody from RABBIT).  I stain decalcified rat spine paraffin sections,

[Histonet] ki-67 staining

Dear all,   I am working on imumunohistichemistry of ki-67 antibody (DAKO MIB-5, a monoclonal mouse anti-rat) on EDTA-decalcifed rat spine and femur.  I used DAKO's reagents such as Dual enzyme block, antigen retrieval solution pH6.0, normal goat serum, anti-mouse Envision plus kit and the DAB k

[Histonet] Decalcification and processing of large bone

Dear all,   I am going to decal and process PIG femur and lumbar vertebrates.  I only have experience on soft tissues.  Anyone can suggest any protocols in decalcification and processing?  Many thanks in advance.   Cheers, Victor ___ Histonet mailin