Hi all, I'm planning to do paraffin embed section on mouse joint (E12~P10) to detect the GFP-tagged protein expression. Since bone has autofluorescence, I'm wondering whether IHC would be a good choice for postnatal bone, and can I do immunofluorescence on joint in E12~16 mouse without having autofluorescence effect?
I've tried cryosection (10 micrometer) but the bone detached and doesn't give me good morphology so I decided to try paraffin. thank you all in advance. W _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
