Hi Carolyn, Our facility currently uses *VWR Chemically Resistant Markers (Cat.# 95042-566)*. I have tried numerous markers over the last 15-20 years and have found these markers to be the most consistant, smudge-free and remain dark after the staining procedure. Oddly enough, certain markers appear to stay on better with different cassettes and different clearants. I have found that if I change to a different cassette, I often have to change markers. Our facility uses Propar Clearant from Anatech Ltd (we don't use Xylene as the clearant) and Simport Histosette 1 (#M490-3). Hope this helps! Tammy
Tammy Weiss Fish Biologist-Histology Dept. Bozeman Fish Health Center USFWS- Dept. of Interior tammy_we...@fws.go <tammy_we...@fws.gov>v On Thu, Apr 21, 2016 at 11:57 AM, Demarinis, Carolyn via Histonet < histonet@lists.utsouthwestern.edu> wrote: > We are having problems with the markers we use, tried several brands, but > they either smudge or are difficult to read after processing. Any > recommendations? Thank you. > > > -- > This e-mail communication and any attachments may contain > confidential and privileged information for the use of the > designated recipients named above. > If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that > any review, disclosure, dissemination, distribution or copying > of it or its contents is prohibited. If you have received this > communication in error, please notify Saratoga Hospital > immediately by e-mail at priv...@saratogacare.org and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet