Hi HistoNet-
I'd love to get any and all feedback about storage conditions for cut slides
and methods use to minimize epitope oxidation. And more specifically, if
anyone is using nitrogen gas cabinets to do so. Another part of the equation
we are trying to look at is defining time periods of
Anita-
We started off with taking them down every other week and moved to once a month
schedule and have had no problems, but you'd want to keep a complete defrost in
mind if persistent sectioning problems ever arise.
Martha
~
Martha Wester
Translational Sciences
MedImmune
Jeff--
We have Leica CM3050's. They have lots of bells and whistles and we've
found that most techs don't make full use of them anyway. But we've
found the Leica product to be solid and have had no real troubles with
them.
Martha
Hi Issac-
Our cryostat decon/defrost procedure goes something like this:
Remove glass door to cryochamber by pulling up and gently sliding out of track.
Allow microtome and chamber to come to room temp. Wipe down exposed surfaces
of cryochamber and microtome with 1% bleach or bleach
