[Histonet] Harvard Brain Bank Job Opportunity

2016-12-30 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: Well, at the age of 67 and after more than 30 years of service, I am retiring from the Harvard Brain Tissue Resource Center (the Brain Bank) at McLean Hospital in Belmont, Massachusetts. My last day is today, December 30th 2016. We are looking for someone to take over my position.

Re: [Histonet] mats

2016-10-05 Thread Wheelock, Timothy R. via Histonet
Hi Mala: We use the following: PIP Technical Contamination Control Mat Vendor: Fisher Catalogue #: 19-122-822 They work very well; just peel off the top layer, and you have a fresh one ready for the next round of sectioining. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA

[Histonet] Salary range

2016-10-03 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: First thank you for letting me know the designation of someone who is certified in immunohistochemistry. I have a second question. Do you have an idea of the average salary range, in the Boston area, for someone who starts a job managing a pathology or neuropathology laboratory

[Histonet] Immunohistochemistry

2016-10-03 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: What is the designation of someone who is certified (registered) in immunohistochemistry? Thanks, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was

Re: [Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Wheelock, Timothy R. via Histonet
Hi Charles: Many years ago I was using deionized water to do the Bielschowsky silver stain. The stains were coming out way too dark, with black-brown precipitate on the sections. When I inherited a double-distilled water filtration system, that problem disappeared. However, silver stains tend

[Histonet] Course of digital imaging?

2016-07-26 Thread Wheelock, Timothy R. via Histonet
Good morning everyone: I have a DP-70 Olympus digital camera. Recently, I upgraded to a cellSens Standard software for the camera. Although some of my images, taken with the old Olympus software were gorgeous, many were not. I am also having problems with the new cellSens software. I feel that I

[Histonet] Over-processing of brain tissue

2016-06-14 Thread Wheelock, Timothy R. via Histonet
Good morning everyone: I seem to be having problems with over-processing of brain tissue. I use a VIP6 processor. I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol. Then half-hour each of three changes of absolute isopropanol. Then half hour each of three changes of xylene. Then

[Histonet] Microscopic slide files

2016-02-12 Thread Wheelock, Timothy R. via Histonet
Hi Rayen: We buy our stackable slide files and slide file base from Fisher Scientific: Here are the items and catalogue numbers, which are for tan-colored files. Other colors are available. Microscopic slide file drawers 07-212-100 Microscopic slide file drawer base 07-212-104 Tim

[Histonet] Favorite book for histotechnology and immunohistochemistry?

2015-12-15 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: I have a co-worker who will be starting in a surgical histology laboratory soon, and wants to get his HTL. What is the best all-around book of histotechnology for him to have? Also, is there a favorite book for immunohistochemistry? Thanks, Tim The information in this e-mail is

[Histonet] Temperature and Humidity check

2015-11-12 Thread Wheelock, Timothy R. via Histonet
Hi: Yes, I check both temperature and humidity as part of a morning " lab systems check". This also includes checking the fume hoods, distilled water, emergency eye wash, refrigerator and oven temperatures, the tissue processor clock, and embedding center and water bath temperatures. Tim

[Histonet] Solvent recycling

2015-11-02 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: My hospital recently has been looking into recycling solvents. Our department alone does not have the volume to justify such an expense. However, there are many research labs on the campus that produce varying (probably small) quantities of solvent. They have asked me to look into

[Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens

2015-08-25 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me

[Histonet] Anonymizing numbering system

2015-08-25 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: Thank you for all your responses and thoughts on this issue. I should clarify that this new system may actually not be coming from the NIH itself, but perhaps from departments in one of our parent organizations. We have no written sources for this sort of approach. They may be

[Histonet] Thank you

2015-08-21 Thread Wheelock, Timothy R. via Histonet
Hi Everyone: I want to thank everyone for the advice concerning the chemical sensors and badges. It is very helpful. Have a great weekend. Tim Wheelock The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error