[Histonet] CMV

Hello All, We are in process of optimization/validation of CMV. Does anyone have any controls to help us in the validation process? I may have some control tissue to trade for another stain. Any help appreciated. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC 423-979-3567 423-979-3

[Histonet] Histonet] Freezing spray in cryostats

We did use freeze spray in the cryostat years ago. Then one fateful day we all had to go on protocol for exposure to TB because a resident sprayed a lung with the freeze spray and there you go. There is a turn around time for frozen sections we are all working with, HOWEVER it is not worth the

[Histonet] Pinning Gross Specimens

Hello all, Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? Lisa White, HT(ASCP) James H. Quillen VAMC ___ Histonet mailing list

[Histonet] Cell Block

Does anyone have the recipe for making cell blocks with Thrombin and Plasma? We made them at my first Histo job, oh say over 20 years ago but I do not remember the ratio. Thanks bunches, Lisa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] : Recycled reagents in VIP processor

We have recycled here for over 14 years. The cost of the recycling unit is not small. It will take a while to recoup the cost. We have found that xylene recycles well. The substitutes are not infinitely recyclable. The biggest savings is that you are not paying to have drums of xylene picke

Re: [Histonet] specimen submission pads

Have they tried not using any pads? Our biopsies (GI, Needle Core, Prostate etc.) are just in the formalin specimen bottle. We don't have any issues with the "free floating" specimens. I have worked at several labs and never seen specimens routinely submitted on a gauze or pad. Just a thought.

[Histonet] Davidson's fixative and IHC question

We have had failure of IHC on CD-20 when fixed in Davidson's. So it is possible. The only thing to do is validate all stains you will use with the Davidson's. Lisa White ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsou

[Histonet] GATA3

Does anyone have a source to purchase GATA3 IHC (+) control slides? Merry Christmas to all the hard working Pathology Superhero's out there for getting the unknowing public quality healthcare for another year J ___ Histonet mailing list Histonet@list

[Histonet] : Are gloves required when cutting FFPE blocks?

I am not aware of any regulation to use gloves to section. However I do use them to section on the microtome, I got really tired of picking human out from under my fingernails. The other 4 HT that I work with do not use them on standard microtome sections. CJD would be the only exception

[Histonet] Microtomy statisitcs

We have a list target of 30 blocks per hour. However we don't track this. There are so many distractions incoming frozen sections, phone calls, drying, loading/unloading a stainer/coverslipper, loading/unloading the IHC stainer, loading/unloading the special stainer, pathologist with questions,