[Histonet] Xylene Substitutes

I know this question has been asked before but what's the best and safest Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cresyl echt Violett for Nissl substance

What is everyone using for this stain? Ruth Yaskovich N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Histonet Digest, Vol 112, Issue 9

Stephanie, This good to know because I'm considering a new microtome. Ruth N.I.H. -Original Message- From: Weaver, Stephanie [mailto:swea...@tvmdl.tamu.edu] Sent: Friday, March 08, 2013 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 112, Issue 9

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 X

[Histonet] Rack for round cover slips

Does anyone have a vendor that has racks that will hold up to 30 to 50 25-mm round coverslips? Vendors welcome. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics ___ Hi

[Histonet] Crystal/Mount

I know this has been asked before. Where can I purchase Crystal/Mount mounting media? Thanks Ruth N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Equipment Repair

Does anyone know of a company that repairs equipment in the Maryland area? I'm in Bethesda and my old Tissue-Tek embedding center won't hold the temperature. Thanks for any help, Ruth Yaskovich National Institutes of Health Bethesda, Maryland ___ Histone

RE: [Histonet] large eye specimens

It's in the Animal Processing Manual from N.S.H. Ruth Yaskovich N.I.H. -Original Message- From: Murray Cumming [mailto:mcumm...@ucalgary.ca] Sent: Tuesday, November 10, 2009 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] large eye specimens Hi Y'all We are looking a

[Histonet] LFB staining with Immuno. Staining

I'm looking for the person who posted this information (I lost the email). I want to stain human and non-human primate ganglia with the TRPV-1 and use the LFB as a counter stain. Any help is greatly appreciated. Thanks Ruth N.I.H. ___ Histonet mailing l

[Histonet] Tetramethyl-benzidine

Has anyone used this chromagen? I want to use it with N52. Thanks for any help. Ruth Yaskovich N.I.H. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] IHC on paraformaldehyde-fixed

We buffer our (4% paraformaldehyde). The animals are also perfused with it. I know I tried to get the investigators to just use the 10% formalin and not make up this stuff from scratch but have just given up. Ruth N.I.H. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED]

RE: [Histonet] IHC on paraformaldehyde-fixed

I do it all the time. Yes your antigen-retrieval method will work. Ruth N.I.H. -Original Message- From: Jan Shivers [mailto:[EMAIL PROTECTED] Sent: Wednesday, December 03, 2008 4:34 PM To: histonet Subject: [Histonet] IHC on paraformaldehyde-fixed Has anyone ever done IHC on parafomald

RE: [Histonet] 200 proof ethanol

I remember working for the Maryland Department of Agriculture and our formalin was pink. I think it was actually Embalming Fluid! Ruth Yaskovich N.I.H. National Institute of Dental and Crainiofacial Research Neurobiology and Pain Branch -Original Message- From: powell_sa [mailto:[EMAIL PRO

RE: [Histonet] Presidential Voting Infomation

I don't think the Histonet is appropriate for this type of Posting! Ruth Yaskovich N.I.H. -Original Message- From: Justin Thomas [mailto:[EMAIL PROTECTED] Sent: Monday, November 03, 2008 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Bar