Dear Histonetters,
We routinely perfuse mice with fixative before taking out organs. Perfusion
with fixative (4% PFA or 10% NBF) is done for only a few minutes (max. 2 min.).
During that short timespan, the entire mouse becomes stiff. Can this stifness
be taken as a sign of good initial
Dear Histonetters,
Does anyone know of a good method to mark (stain) small samples (tissue or
cells) prior to paraffin embedding to aid in finding back more easily the
sample in the paraffin block ?
Preferably something that would not interfere with subsequent antibody staining.
Yves
Dear Histonetters,
Is it technically possible to perform a double staining using alkaline
phosphatase in both stainings ?
I was thinking to perform a seqeuntial staining and develop the staining for
the first protein and detect with a blue AP substrate and then perform staining
for the second
Dear Histonetters,
I am trying to combine a peroxidase-DAB staining (brown) with an alkaline
phosphatase-staining for detection of two proteins that do not overlap.
I have already tried Dako’s Permanent Red and Fuchsin+ and Vector’s Vector Red
as AP substrates but I am not getting a clear red
Dear all,
Is there any (published ?) negative effect when tissues stay in hot paraffin
over the weekend on antibody staining (diminished staining intenstity ?) ?
Yves
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