[Histonet] What kind of undecalcified bone, mama or frozen, human or mouse?

Message: 1 Date: Tue, 19 May 2015 17:01:04 + From: "Hernandez, Jesus Willibaldo" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, I just wanted to know if any

Re: [Histonet] Histonet Digest, Vol 138, Issue 24

Where are the photos? Can we upload photo? > On May 20, 2015, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthw

[Histonet] Re: Post fixing IHC slides

Hi Cecilia, Glad to know post fixation will reduce section falling off from slide. We are bone research lab and always have problem of paraffin bone section falling off after antigen retrieval. There are many ways we have tried, but not improve too much. I know bone section will be easy to fall

[Histonet] Alk pho staining on paraffin section

Hi, I couldn't do on paraffin tissue after trying a few times. But works fine on MMA plastic section. If any one has a practical protocol, please share. Thanks in advance. Dorothy Hu Hello, I was wondering if anyone has had success in staining osteoblasts via alkaline phosphatase in decaled

[Histonet] Why when I submit my answer to the list an email with Korea language pop up?

Can it be blocked? Thanks. Dorothy Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryosectioning undecal none

Hi, We are using Kawamoto's Section-Lab Co. Ltd. products now. It is better than cryojane. Check Kawamoto's paper online to get protocol. Good luck. Dorothy Hu HSDM Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://li

[Histonet] decalcifying sheep knee joints

Hi, You may use formic acid instead of EDTA, it will be much fast and give you sharp image of Safranin O. You can also do IHC. Dorothy Hu HSDM Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/

[Histonet] TRAP staining for ffpe sections

Hi, I make staining solution for TRAP staining. Used once Sigma kit, but unsatisfactory. You may Google for TRAP protocol online. It must be there and easier than the kit. Dorothy Hu Manager of histology and histomorphometry operation HSDM Sent from my iPad ___

[Histonet] Vascular contrast agent injected tissue

Hi histoneters, Does any one out there to process tissue in a tissue process machine after lead or barium agents introduced to your tissue? It will do any harm to the machine? Get clog or contaminate other tissue? Thanks for your help. Dorothy Hu MGH endocrine histocore Sent from my iPad >

[Histonet] Tissue falling off

Hi Susan, I was so glad that I saw your post regarding tissue falling off. We have had this problem for a while, especially for human bones tissue. This prevents us to do any heat induced antigen retrieval. If it possible to email me how do you mix soapy water with ammonia? How many ml of water

[Histonet] Can I use no water benzoyl peroxide from PolySciences in my MMA mixture?

I try to avoid CaCl drying step in making MMA infiltration and embedding solutions. Please help me MMA experts. Many lab use benzoyl peroxide which has 30% water in it. And go through CaCl afterwards. Another question, why some lab use Butanol and toluene before coverslip? Why use Cellosolve to

[Histonet] Re: pdf of JOH publication attached

Hi Gayle, Thank you very much. Please send me the paper of perkadox from Stain Technology. We try to determine benzoyl peroxide or perkadox to do mouse undecalcified bone in MMA. Any previous people's study regarding this topic will be greatly appreciated. Dorothy Sent from my iPad > On Jan

Re: [Histonet] Re: Do I have to destabilize MMA?

Thank John very much. I will check out your references. Hope we are not only be able to follow the protocol, but fully understand it. Dorothy Sent from my iPad > On Jan 16, 2014, at 1:37 PM, John Kiernan wrote: > > Methacrylate monomers are sold with added hydroquinone to inhibit spontaneous

[Histonet] Do I have to destabilize MMA?

I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to