We do not use AE1/AE3 anymore. OSCAR single clone is a much better antibody. We
retired AE1/AE3 out of fear that it will lead to false negative results in
sentinel nodes.
Hadi
On Oct 3, 2012, at 3:50 PM, Jackie Ferracone wrote:
Nice antibody, we use Proteinase-K, at a dilution of 1:500.
I agree with Linda. One positive control per *batch*, not per case. The
positive control tissue can be placed on one of the patient's specimens, which
saves you money on the detection system and antibody as well.
We should be careful also about treating different drawers with different runs
When we started our lab 3 years ago, we began with SP1 from day 1, so
I don't have any experience with either 1D5 or 6F11 except in my
previous labs. 1D5 is an excellent clone, and seems to be more
specific than SP1 in the work-up of metastatic carcinoma of unknown
primary site, based on
