1. 30% H2O2 5 min. under movement.
2. wash in water 2x
3. 5% Acetic acid 1 min.
4. wash in water 2x
5. solution 1 20 min.
6. wash in water 2x
7. solution 2, piece by piece
8. wash in water and dry.
Solution 1: 1 part Azur II (1%) + 1 part Methylen blue (1%) + 2 parts
Na2CO3 (1%).
Solution 2: P
Hi James:
We work with technovit 7200 VLC and use Silicoup (kulzer) as
pretreatment for the glass slides. The blocks must be completely dry
(I leave one day drying in the lab without working on it).
Fernando Muñoz
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