We follow the same process as Jeff.
Michelle
On Feb 24, 2015, at 4:59 PM, Jeffrey Robinson
wrote:
HI Renee- I had a major problem in the past with tissue staining for IHC
lifting off the slides no matter what I tried. It turned out that when we
precut our controls and put them in the ove
I agree with Sheila. At our place, it was fire code that everything be off the
ground by 6"... not just blocks, but anything being stored.
Michelle
Sent from my iPhone
On Feb 9, 2015, at 1:30 PM, Tapper, Sheila J.
wrote:
In my experience, this is usually a local fire code requirement.
Shei
We provide assistance, primarily on the outpatient frozens, but help with
in-patient ones as well.
What makes you ask?
Michelle
Sent from my iPhone
On Feb 6, 2015, at 10:37 PM, "lsmal...@juno.com" wrote:
Hi, I need to ask Histoland a questionHow many HT departments provide
assistance to
We also use the para-trimmer. In my view, it is worth its weight in gold! I
can melt 5 blocks at a time, works like a charm. I am one who does not mind
the wax on the sides, as I am most confident that there is enough paraffin to
support the cassette.
Michelle
Sent from my iPhone
On Jan 21,
Please disregard
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
I second the Leica XL. That stainer is a reliable workhorse.
Sent from my iPhone
On Jan 7, 2015, at 8:08 PM, ian bernard wrote:
The Leica Autostainer XL has proven effective for our lab.
IRB
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@
Hello Histonetters :)
With regards to billing for multiplex IHC stains, I have a scenario and
question.
Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is
done on both blocks A1 and A2. Stain2 is done on block A1.
How would we bill for this? Would it be 88344X2 - becau
Once the block has been faced, we melt the excess paraffin from around the
tissue, being careful not to seal the exposed tissue in paraffin. We remove the
tissue from it's embedded cassette, put it in a newly numbered cassette and
place it in the processor for reprocessing.
Works well in our l
eless BlackBerry
>
> -Original Message-
> From: Jesus Ellin
> Date: Mon, 8 Oct 2012 23:40:37
> To:
> Cc:
> Subject: Re: [Histonet] Facility Name on Slides
>
>
> To my knowledge there is no regulation rather than having 2 identifiers,,
>
> Sent f
Does anyone have any regs that you can point me to for both Joint Commission
and CAP which details definitively whether or not a facility name is *required*
to be included on slides?
I know it's a good idea, the larger question here is whether or not there is a
specific regulation for it?
Tha
We have two back ups! :)
We also have back up tissue processors and microtomes as well.
Sent from my iPhone
On Sep 21, 2012, at 8:15 AM, "Manfre, Philip" wrote:
> If you use anything often enough, it is wise to have a back-up, if possible.
> Microtomes, and especially cryostats, tend to malf
Here are some links for HT/HTL licensure in FL.
http://ww2.doh.state.fl.us/mqaservices/PractitionerServices.asp
http://www.doh.state.fl.us/mqa/ClinLab/clp_applications.html
There is a lot of information on that page, not just the application, so you
might want to spend a few extra minutes
Does anyone know of a company who buys used Histology equipment and would give
quotes for used Milestone Histos 5 microwave tissue processors.
Thanks!
Michelle
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/m
Please see the following link for more information and online application.
https://uhs.ats.hrsmart.com/cgi-bin/portal/highlightjob.cgi?jobid=75412
Manatee Memorial Hospital, a 319-bed acute care facility is located on the
banks of the Manatee River in Bradenton, Florida. The hospital has been a
We use Aldex, neutralize the formalin and dispose of the neutralized gel in the
biohazard trash. The manufacturer says it can go in regular trash, but we
choose to put it in biohazard trash.
Michelle
On Aug 17, 2012, at 4:18 PM, Vanessa Perez wrote:
> We use formalex green.
> http://america
Is Cyto prep considered a high complexity task?
On May 17, 2012, at 12:59 PM, "Yang, Mari" wrote:
> Sheila,
>
> Maybe the inspector was referring to the prep? Many cytology labs use
> automated staining. If you find out any information, I'd love to know.
>
> Thanks,
> Mari
>
> Mari Yang, MH
ke a coverslipper to use with their labels or
> change their labels to something thinner. Symphony takes too long to use as a
> coverslipper.
>
> >>> "histot...@imagesbyhopper.com" 5/21/2012
> >>> 3:30 PM >>>
> We have the CV5030 and have had *m
We have the CV5030 and have had *many* issues. I have a graveyard of the
composite racks with broken "ears" on them. The machine has broken racks and
slides, thrown coverslips, dropped slides and we kept being told that we just
needed adjustments. Later we were told that a new electronics board
I like the StatLab markers.
On Dec 3, 2011, at 1:38 PM, "Rathborne, Toni"
wrote:
Can anyone recommend a marker for using on cassettes? We currently use pencil,
which sometimes smudges. We've tried a few markers already, but some fade,
while others hold up well for processing, but won't when
Wow. Just Wow! :o(
Sent from my iPhone
On Oct 5, 2011, at 7:03 PM, Jenny Vega wrote:
> Ok I want to know the diferrence between Neutral Buffered Formalin 10% and
> NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same
> thing and in the book from Frieda Carson it says that
We use the Leica autostainer, bridge & coverslipper. We use the premimum
coverslips from Fisher and seldom have any issues with them sticking together
(and we're in FL!)
Does anyone have issues with the coverslipper breaking off the "ears" on the
grey/black racks? I have a cupboard "graveyard"
Interestingly, I have never been required to section tissue in a job interview.
I have worked at four hospitals, three private labs and one research facility
(hubby moved us around a bit!)
I just recently hired two techs, directly out of school with no real world
experience. I did ask them to c
Way to go Patsy!! :o)
Sent from my iPhone
On Aug 29, 2011, at 5:36 PM, "Patsy Ruegg" wrote:
> Hey Everyone,
>
>
>
> The third tech I trained in the last 3 years just passed her HTL exam last
> Friday, that all makes me feel pretty good. I just signed an affiliate
> agreement to be a clinica
10 years experience to qualify for taking the exam? Is that an ASCP
requirement? It sounds like they might be exchanging experience for the
bachelor's degree? Have you checked out the online courses? I believe there
are some that offer the bachelor's degree.
Michelle
Sent from my iPhone
O
Sorry, I misread, the experience must be within the last 10 years.
Michelle
Sent from my iPhone
On Aug 27, 2011, at 1:45 PM, "Lee & Peggy Wenk" wrote:
> "It looks like the time limits prohibit me from trying this, since I did it
> so long ago"
>
> What is "IT"? What is prohibiting you from t
:o). I'm not even sure I could find my results after all these years and 9
moves!
I *do* remember how picture perfect those slides had to be though. Tiny air
bubble=graded down. Folds=forget it. Knife marks=bad.
I did both the HT and the HTL practicals and now new students don't even have
to s
Liquinox and bleach soulution works for us.
Michelle
Sent from my iPhone
On Aug 26, 2011, at 6:24 PM, "Jeter, Brent"
wrote:
> Histonetters,
>
> Can anyone recommend a good cleanser for soaking scissors/blades/instruments?
>
> Thanks in advance -
>
> Brent Jeter
> Anatomic Pathology Supervi
Is it a CAP requirement to have the facility name on the slide labels?
We have a friendly bet going on the answer to this one! :o)
Thanks in advance!
Michelle
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://l
Statlab pens for both cassettes (when we hand write them) and for slides.
Michelle
Sent from my iPhone
On Aug 22, 2011, at 11:26 AM, "Sherwood, Margaret "
wrote:
> We use the marking pencils, exlusively, for our cassettes and StatLab marker
> pens for our slides.
>
>
> Peggy Sherwood
> Lab
Good gosh, don't tell that to the reference labs who routinely send out
finished slides!!
Sent from my iPhone
On Aug 10, 2011, at 5:30 PM, b427...@aol.com wrote:
>
> Can someone provide a quick reference to ANYTHING I can use to prove to my
> EHS that fixed, processed, coverslipped slides ar
I agree, the Para-Trimmer is the next best thing since sliced bread! I'll
never go back to the knife again.
We use lid from one of the Ventana special stains boxes to catch the wax. I
have also fashioned a drip tray out of the cardboard boxes that the coverslips
come in.
We're all McGuyver's
Ruthie,
In order to unsubscribe from the list, you must click on the link at the bottom
of the email, scroll to the bottom of the webpage and enter your email
requesting to be unsubscribed. You have the power to unsubscribe yourself!
Good Luck!
Michelle
Sent from my iPhone
On Jul 26, 2011, at
Hi Histonetters!
I'm looking for thoughts on preferences/pros/cons between using a progressive
and a regressive H&E on routine daily work.
Which hematoxylins do you prefer (commercially prepared), which eosin?
Anyone have a tried and true protocol for each method?
Thanks!
Michelle
Sent from
Sharon,
We use a product called Aldex to neutralize our formalin. It binds with the
aldehyde groups and neutralizes them. According to the mfr, you can dispose of
it in regular trash, but I am more cautious and throw it away in red bag trash.
Michelle
Sent from my iPhone
On Jul 22, 2011, at
Hi Histonetters!
For those who use recycled alcohol, I have a few questions.
Do you use it in your automatic H&E stainers?
Are you having any troubles with proper, consistent H&E staining?
We are using recycled alcohol, and wish to continue to do so IF we can. We are
having issues with our sta
We do, on average, about 15 autopsies per year. We have a diener who does them
for us. The pathologists are present and are often are hands on during the
autopsy. Sometimes a lab aide will be there in the mode of a scribe. Our
histotechs do not participate in autopsies.
Michelle
On Jul 20,
Have you tried drying them under a "blow dryer"? That's how we used to dry our
slides before we sent them out. It was a commercially available slide dryer.
Michelle
Sent from my iPhone
On Jul 14, 2011, at 3:57 AM, "Gudrun Lang" wrote:
> I think it's more depending on the organic solvent used
CONGRATS!!! :-D
Sent from my iPhone
On Jul 11, 2011, at 12:08 PM, wrote:
> So I got married and my name changed (meaning so did my email) tried
> sending out a broad email, but it didn't work. Just making sure I'm
> updated now =)
>
> Happy Monday all!!
>
>
>
> Sarah Goebel-Dysart, BA, HT
Amita,
The paraffin that we use is also a Leica product. It was formerly sold under
the Surgipath label, but since to companies have merged together, it now sells
under the Leica name. It is a "beaded" pellet paraffin and has a version
suitable for infiltration and a version suitable for embedd
For those who use the Ventana IHC and special stainers, how do you dispose of
the liquid waste that is generated?
Thanks!
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
We use the infiltration & embedding paraffin from Surgipath/Leica and are very
happy with it.
Michelle
Sent from my iPhone
On Jul 8, 2011, at 12:44 PM, "Paula Lucas" wrote:
> Hello,
>
>
>
> We are considering a switch to a different brand of paraffin and this is
> because I feel we are hav
in your MW processor, unless your provider wants
> you to buy your 2-propanol from them.
> René J.
>
> --- On Tue, 7/5/11, histot...@imagesbyhopper.com
> wrote:
>
> From: histot...@imagesbyhopper.com
> Subject: [Histonet] Isopropyl alcohol
> To: "Histonet@Lists. Uts
We recycle our isopropyl alcohol. We have been told by our microwave
manufacturer that we cannot use the recycled alcohol on the microwave processor.
As a result, I am trying to find additional uses for the recycled isopropyl.
Is there any reason why I can't use the iso in place of ethanol when
I second the Leica! :o)
Michelle
Sent from my iPhone
On Jun 29, 2011, at 1:14 PM, "White, Lisa M." wrote:
> We use the Leica CM1850 UV. It is easy to use for sectioning as well as
> UV decontamination. Pricing fell in line with other vendors. I have
> used Shandon, Leitz, Leica and a unit
OMG, 100 blocks/hour? Seriously? I am fast , but even I can't touch that!
That's simply insane!! :o(
Sent from my iPhone
On Jun 28, 2011, at 6:20 PM, "Grantham, Andrea L - (algranth)"
wrote:
> I'd do it - along with Rene's paper which was published and the Canadian
> paper that was ref
To ensure that I am understood correctly: I agree that the expectations for
Joanne are unreasonable and unfair.
I have read here some replies that more acurately explain what I thought better
than *I* did!
- Speed (while maintaining quality) will come over time. One *must* have
quality or spe
Not to add fuel and all that... but I can, and regularly do, cut single slide
blocks in less than minute per block. I am not a new tech though, been around
the microtome a few times!
I think the proper approach would be to have the tech *average* the number of
slides the cut over a period of
And remember mouth pipeting? "oops, that's the cotton..."
Eating and smoking in the lab was the norm.
Our alcohol had the tax stamp on it! :o)
Michelle
On Jun 19, 2011, at 4:19 PM, Paula Sicurello wrote:
> Sheesh! We used to have people smoke while working with propylene oxide.
>
> Eating
Dorothy,
Is your question related to the fact that a protocol will have the "currently
active" lot number and you want to use a different lot number? Or is this a
brand new antibody that has not been in use before and therefore has not ever
been validated?
Michelle
On Jun 15, 2011, at 11:41
:-D @ Linda!
Fun thread Sara! :o)
Michelle
On Jun 14, 2011, at 2:58 PM, "Blazek, Linda"
wrote:
> And we all can tell you are creeping nearer and nearer that non pathologic
> (al) island oasis in the toluidine blue sea of retirement. Periodically I
> think of that time but it's not near e
I have kids going to, or getting ready to go to, college. I sure don't see
retirement in my near furture!!
Michelle
On Jun 14, 2011, at 3:30 PM, wrote:
> I'm 30, so blah blah blah...I have 30 or 40 more YEARS!!
> =)
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Thera
We don't keep it off the floor, but do use a wide-bladed putty knife attached
to a mop handle to scrape the residual wax off the floor. It woks quite nicely
and doesn't remove the actual floor wax like a razor blade scrapper would.
Michelle
Sent from my iPhone
On Jun 14, 2011, at 6:44 PM, JR R
We do micros on every one of them.
On Jun 3, 2011, at 12:13 PM, "Horn, Hazel V" wrote:
> Generally all are gross only. There are some exceptions depending on patient
> history or if one is significantly larger than the other the pathologists
> have the discretion to do a microscopic.
>
>
We currently use paper reqs, but coming soon, we will be going
paperless. I am not sure I am going to like this... ;o). Surgical staff will
enter the "req" in the computer, we receive the order & the specimen and enter
into our AP system.
There are going to be growing pains!!!
Sent from my
For the keyboard, what about using the silicon style? A lab near me uses them
exclusively. They are easy to keep clean, as they basically watertight - just
wipe down with a sani-wipe! And, they are fairly inexpensive - I got one from
Office Max (or was it Staples?) for $10.
Sent from my iP
We had a similar thing happen last week, except in our case it was the eosin
was very pale & the hematoxylin was more blue than purple. Through our
troubleshooting steps, we determined it could have only been the processor
reagents. We fully changed it and the problem went away. While I am del
We had a patient we named Oscar Meyer... it was a piece of hot dog that we put
in formalin, the doc grossed it, we cut it (and it cut very well, I might add!)
and they got to the point of looking under the scope before they realized the
joke! ;o)
Michelle
On Mar 29, 2011, at 2:13 PM, "Breeden
Hi Histonetters!
Can anyone tell me (maybe even provide a req) as to whether or not a lab aide
can run the Histos 5 microwave tissue processor? It's semi-automatic, which
involves the person (lab aide, tech etc) moving a carousel of cassettes from
one beaker to another.
Any help is greatly ap
It is my understanding that you may now charge for every stain, even if it's
two blocks from one "bottle". The CMS requirements changed in Oct of 2009, if
memory serves correctly. Perhaps some one else can site the reference or go
back into the archives for it. I'm not at my computer, or I wo
Another place to check is the "holding" water that the slides rest in just
prior to staining. We run our slides to water on the H&E stainer and then
transfer the rack to a "holding" water dish. We bleach this dish nightly, as
we have found it contaminated in the past. We don't know what cause
I have a run set up to bake the slides & run them down to water ( for my
specials), another just to start at hematoxylin down to xylene etc. The
stainer allows for nearly any custom program you can think of. :o)
If you have any questions about how to set the program up, please let me know &
I
We process our bone marrow biopsies the same day we receive them. Specimens
are received in formalin for the core and B+ fixative for the clot. The core is
placed in decal solution for a fairly short time (30 minutes or so) and then
routinely processed. As a routine, at the microtome, core bi
Our method isn't as technology savvy, but it works well for us. All blocks
submitted for processing are written down on our "piece sheet". At the end of
the grossing day, two staff members compare the blocks in the processing basket
to those listed on the piece sheet. At embedding, the histotec
I have a tech who complains about static/compression in our cell blocks, but I
don't seem to have the same problem as her. The difference? I face my blocks
and then soak it on my waterbath for about 15 -30 seconds and then it goes onto
the ice tray. The additional moisture that is soaked up in
Oh man! The first time Histonet stopped working, I thought it was my email...
but this time I knew better! And I too didn't realize how much I looked
forward to the messages/thoughts/questions shared on this board.
So histonet, what's been causing the problem?
Michelle
On Dec 17, 2010, at 1
Hi Meghan,
We use grey colored boxes that will hold either 8 cardboard "drawers" of blocks
or 4 cardboard "drawers" of slides. I am at home right now, & don't have the
specific ordering information, but will share it tomorrow when I get to work.
I just didn't want to let the question languish
Not in Florida either. We use Aldex to neutralize the formalin & then dispose
of it on the biohazard trash. The mfr says once neutralized you can put it in
regular trash, but we don't.
Michelle
On Nov 11, 2010, at 7:45 PM, jmacdon...@mtsac.edu wrote:
> Absolutely not here in southern Califo
We have a calendar book in the frozen section room. The pathologist places the
patient label on the book on the appropriate date, writes both the start & stop
times. I don't count the multiple block ones, but I don't tell them that! ;o)
If they stay in the habit, all the better for me! Oh ye
Allison,
I have created an excel spreadsheet where you put time started, time completed
& it will calculate the amount time spent doing the frozen. It highlights any
time grater than 20 min. Let me know if you are interested in something like
that.
Michelle
On Nov 1, 2010, at 2:54 PM, "S
It sounds like I am in the minority in pitting the eosin in the *first* 100%
alcohol!
I agree with Bill, it really makes a HUGE difference in small, minute tissues.
We did run into one issue though, we were using orange biopsy cassettes and the
red-orange tissue was difficult to spot in the
We use about 40ml of eosin in the first 100% alcohol in both of our "large
specimen" & "small biopsy" machines.
Michelle
On Oct 21, 2010, at 4:33 PM, "Scott, Allison D"
wrote:
> Hello to all in histoland. Are any of you using eosin on the processor
> to dye your small bx's? If so, are you
Statlab pens for us. We have a cassette printer, but we hand write our slides.
I *love* the statlab pens!!
Sent from my iPod
On Oct 19, 2010, at 5:26 PM, "Sherwood, Margaret "
wrote:
> Victor,
>
> We are a research lab and don't generate near the amount of specimens that a
> clinical lab w
Can you please provide the specific CMS update number? The website doesn't
seem to be too user friendly ... thanks!
On Oct 14, 2010, at 12:22 PM, "Weems, Joyce" wrote:
>
> CMS/NCCI Update Dated October 1, 2009
>
> 8. The unit of service for special stains (CPT codes 88312-88313) and
> i
Sean, what state are you in? If FL, there is a company called Southern Biomed
who could probably help you out.
Michelle
On Sep 29, 2010, at 10:28 PM, "Sean McBride" wrote:
> Hello everyone,
>
> I have an old Leica RM 2065 microtome that is in need of repair (the
> clutch stopped working),
Unless someone corrects me (or even agrees with me!) in FL only a technologist
is allowed to load the IHC machine, so no additional lean for us. :o(
I would be interested to hear more about the savings though, as we are
preparing to be in the market for a new IHC machine. We currently have the
We cut 10 unstained slides as well.
Michelle
On Aug 26, 2010, at 6:19 PM, "Rae Staskiewicz" wrote:
> We cut 10 unstained slides.
>
> Rae Staskiewicz
> Methodist Medical Center of Illinois
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun
We have the new version of the Leica XL stainer (looks just like the
old one!) with the bridge-transfer station & coverslipper & love it.
We had the old stainer for 10 years before getting the new one... and
even managed to sell the old one!
Michelle
On Jun 30, 2010, at 9:57 AM, "Webb,
We have very good success in facing the block and soaking it on the
water bath for 30-60 seconds and then allowing it to cool down on a
very wet ice tray. Ours cut like butter too! :o)
On Apr 28, 2010, at 8:58 AM, "Cazares, Ruth"
wrote:
Or you could soak them in 10% sodium hydroxide f
the help!
Michelle
-Original Message-
From: Lynette Pavelich [mailto:lpave...@hurleymc.com]
Sent: Tuesday, April 27, 2010 6:56 AM
To: histot...@imagesbyhopper.com; histonet@lists.utsouthwestern.edu;
MARY
HODGES
Subject: RE: [Histonet] Bone Marrow Clots - falling off slides
When I start losi
79 matches
Mail list logo
