[Histonet] Input on Sakura E150 Purchase

2014-02-26 Thread margaret chadwell
, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla

Re: [Histonet] Frozen IF Specimen Storage: Is -80 C really necessary; will -20 C work?

2013-11-11 Thread margaret chadwell
Hi David, An institute that I work for would take the samples out of the -80 and place them into a -20 before I cut them, sometimes this would be overnight other times several hours. We were experiencing freeze thaw artifact on those samples, when asked why they were doing this, they thought

Re: [Histonet] Looking for cryomolds

2013-07-29 Thread margaret chadwell
Hi Carol PSL (Pacific Southwest Lab Supplies) sells the round Cryomolds. Margie Chadwell Sent from Windows Mail From: Carol Torrence Sent: ‎Monday‎, ‎July‎ ‎29‎, ‎2013 ‎9‎:‎18‎ ‎AM To: histonet@lists.utsouthwestern.edu I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab.

[Histonet] Cutting Frozen Brain

2013-07-11 Thread margaret chadwell
Hello Everyone, Does anyone have any suggestions for cutting corneal rat brains (20 microns), fixed in PFA, treated with sucrose and embedded into OCT. They cut well but when I pick them up on the slide they always have wrinkles, I have tried many different things with no luck. Different modes

Re: [Histonet] HPV ISH Probes

2013-06-26 Thread margaret chadwell
We have gotten good results with the following probes. http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent.

[Histonet] Autotechnicon processor

2013-05-31 Thread margaret chadwell
Does anyone have an Autotechnicon Tissue Processor for sale?Thanks much!Margie Chadwell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet