[Histonet] Histoline company

2016-06-12 Thread mohamed abd el razik via Histonet
Dear All we have a limited fund for buying new automatic microtom. we have got offer from an agent for model RM3600 histoline company - Italyhave any one worked befor with such model or any recommendations please thanks ___ Histonet mailing list

[Histonet] Rabbit and chicken eye processing

2016-02-25 Thread mohamed abd el razik via Histonet
Dear all What is your optimal protocol for processing rabbit and chicken eyes ? and do you prefer to cut it 2 halfs or inject it with NBF 10% or whole processing is the best ?? Thanks in advance Mohamed ___ Histonet mailing list

[Histonet] clearing time

2016-02-04 Thread mohamed abd el razik via Histonet
Dear all what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ? thanks Mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] unsolved proplem

2016-01-26 Thread mohamed abd el razik via Histonet
Dear allI have submitted a proplem about reprocessing tissue blocks that have patches of stained areas and unstained pale yellow areas (H stain)!!!, unfortently the proplem didn't solved yet and i have tried all possible solutions, increasing processing time and ordered new chemicals from other

[Histonet] tissue reprocessing

2015-12-18 Thread mohamed abd el razik via Histonet
Dear all I have couple quesions please 1- is there a protocol for reprocessing of mice kidney and liver after paraffin embedding due to insufficient processing time? 2- Is there a formula to add to processing agents as softening agents? thanks Mohamed

[Histonet] curling of kidney sections in water path

2015-12-10 Thread mohamed abd el razik via Histonet
dear all, i'm facing an issue that trouble us when we process tissue samples of different organs and cutting them; all organs are ok and spread fine and somoothly on ater path except for kidney sections (looks wrinkled and curled as if the praffin fram around it prevent it from spread!!!). i

[Histonet] paraffin dispenser help!

2015-08-31 Thread mohamed abd el razik via Histonet
Hi all i'm in urgent need for operating SHANDON LIPSHAW PARAFFIN DISPENSER MODEL 224 how to start using it please in simple stepsthanks in advance mohamed ___ Histonet mailing list Histonet@lists.utsouthwestern.edu