[Histonet] Drivers for AxioCam MRc 5 from Zeiss

Dear histonetters, I am looking for drivers for the AxioCam MRc 5 from Zeiss. Unfortunately Zeiss webpage is not very helpful. Thanks in advance Pablo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mai

Re: [Histonet] Over- decalcified tissue

As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint. Pablo Sanchez Laurie Colbert escribiu: I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there

[Histonet] Eosin pH

Listers, Could you tell me the best way to adjut the eosin pH to 4-5? Thanks in advance Pablo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Masson trichrome and H and E

>>At 19:19 03/03/2015, you wrote: >>Hi! >>For lacking Eosin-staining check the pH of the solution. It should be 4-5. >>Gudrun >>= = = Thanks for the tip! Nice to know it... after centuries doing stainings :-) Pablo On 03/03/15, Emily Brown wrote: > Hello! >

[Histonet] Chromic Acid for Cleaning Glass

Dear Listers, I think that the use of Chromic Acid for cleaning the glassware is currently declined. Do you keep using it? Could you suggest me a non-comercial alternative? Thanks in advance, Pablo (Spain) ___ Histonet mailing list Histonet@lists

[Histonet] Cutting Tendon

Dear listers, I am trying to get transversal sections of cow flexor digital tendons. I need sections of the whole piece, about 1cm diameter. Before paraffin embedding I have treated the samples with: -KOH 4% -4% phenol in 70% ethanol In both cases the r

[Histonet] Help cutting horse tendon.

Dear listers, I am trying to cut whole sections of the tendon of the digital flexor muscle of the horse. The piece is around 1 mm long and very elastic and fibrous. I have tried formalin and Bouin fixation, the latest better. With paraffin embedding the fibrous elements of the tendon spoil th