[Histonet] time for penetration of methacarn fixative

Does any one have data for the time it takes for methacarn fixative (60% methanol, 30% chloroform, 10% glacial acetic acid) to penetrate and then fully fix tissue? PW Noyce -PhD student ___ Histonet mailing list Histonet@lists.utsouthwestern.edu htt

[Histonet] RNA preservation in Farmers solution

How long can tissue be stored in Farmers Solution (ethanol + acetic acid) before the RNA degrades. PW Noyce PhD student ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Toluidine blue stain fo plant tissue

Could anyone give me the concentration (? 0.1-0.05%) and the exact technique/protocol to use. I am staining shoot apical meristems not leaf or root. Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://list

[Histonet] plant tissue stain

This may seem a bit odd for this forum but what is the best stain for plant tissue histology slides-to show nuclei, cytoplasm, cell wall. PW Noyce. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listi