[Histonet] Orienting GI biopsies during embedding

2009-09-24 Thread ssylvest
Everyone, We are having a heck of a time orienting GI biopsies but esophageal biopsies in particular, during embedding. If anyone could share their secret for getting these tiny specks of semi-transparent tissue oriented, we would greatly appreciate it. Sabina Sylvest Department of Pathology

[Histonet] Procedure for anti-human TCR V alpha24

2009-08-24 Thread ssylvest
Hello all, I am working in an Allergy and Immunology research lab and we have been trying to work up a flow cytometry antibody for IHC on paraffin. The antibody is mouse anti-human TCR V alpha24-Biotin from BeckmanCoulter. Does anyone have a working procedure for this antibody? I have tried