Re: [Histonet] Xylene substitute for processing GI biopsies

2014-04-21 Thread Rene J Buesa
s.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can

RE: [Histonet] Xylene substitute for processing GI biopsies

2014-04-20 Thread Joe W. Walker, Jr.
nt: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher s

SV: [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Karen Inge Nielsen
...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] på vegne af Lori Gemeinhardt [loreli...@mac.com] Sendt: 18. april 2014 02:18 Til: histonet@lists.utsouthwestern.edu Emne: [Histonet] Xylene substitute for processing GI biopsies Hi there! I have spent a lot of time reading the archives, but still

Re: : [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Teri Johnson
Hi Lori, Pretty much any xylene sub will work for processing tissues and staining. You may need to add additional time in the "xylene" steps, since the action of the subs isn't as quick as xylene. In addition, the substitutes are more intolerant of water. How humid is your lab? If it's dry, the

RE: [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Elizabeth Chlipala
: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, April 18, 2014 10:41 AM To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsie

RE: [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Tom McNemar
tonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with

RE: [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Blazek, Linda
n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known

Re: [Histonet] Xylene substitute for processing GI biopsies

2014-04-18 Thread Rene J Buesa
Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coversli

[Histonet] Xylene substitute for processing GI biopsies

2014-04-17 Thread Lori Gemeinhardt
Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad