Hi Gabrielle,
Are you doing floating sections? Did you cut the tissue recently? Do you
notice your tissue is also "sticky"? If you do, there is a possibility that
the tissue may be infected by bacteria. Try washing the tissue with PBS or
TBS with 0.1% sodium azide a couple time. Sometimes it
To whom it may concern,
I am a undergraduate researcher at Vassar College. I am performing
immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG
(1:200),ABC (Vector labs) and DAB. So far we have gotten really great
staining, however the last immuno I ran, the sections, once
