I assume that the fresh tissue was fixed in methanol?
If so, it is irreversibly coagulant- fixed ( like boiling an egg white...no
going back)
Place the specimen in dist water until it melts to RT
x3 changes of dw 1hr each at RT on a gentle rocker to get rid of methanol (
antifreeze, hence
onet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryotomy help
Once the tissues are in any ethanol or methanol you will never be able to
get a frozen section. The only way I have been able to salvage anything
like this was to place the OCT block into 10%BNF on a rotator and let them
thaw/fix and pro
t Dept of
>> Ophthalmology
>> 7.373 UPMC Mercy Pavilion1622 Locust St.,Pittsburgh PA 15219
>> (412) 624-8508 this number cannot receive texts
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>> -Original Message-
>> From: Charles Riley via Histonet
>> Sent: Friday, July 12, 2024 12:04 PM
&
1622 Locust St.,Pittsburgh PA 15219
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>
> -Original Message-
> From: Charles Riley via Histonet
> Sent: Friday, July 12, 2024 12:04 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Cryotomy help
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onet@lists.utsouthwestern.edu
Subject: [Histonet] Cryotomy help
I have a researcher who is trying to mass spectrometry on frozen tissue
sections. They submitted the sample in methanol and even after sitting in a
-80 freezer overnight the samples are too soft to section.
Does anyone have any
I have a researcher who is trying to mass spectrometry on frozen tissue
sections. They submitted the sample in methanol and even after sitting in a
-80 freezer overnight the samples are too soft to section.
Does anyone have any tips on how to harden the tissue for sectioning that
won't damage the
