Hello all, I noticed CST sells most of their antibodies with 50% glycerol and recommends -20C storage and no aliquoting. They remain in liquid form in the freezer. A little bit more difficult to pipette precisely due to the viscosity and temp difference, but it seems like a good trade-off for easy handling and storage. Some other manufacturers also supply with 40% or 50% glycerol, but only for a few antibodies and it isn't common.
Has anyone experienced problems with their antibodies from adding glycerol 1:1? I know they shouldn't be more than 1% or so in the final working solution, but given that most IHC antibodies are diluted 1:100 or greater, this doesn't seem to be an issue. I tested on a few antibodies I had and saw no difference in staining, but I haven't done long-term testing yet. I'll probably add a little bit more sodium azide too. Thanks, Allan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
