Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids.
Bernice
-Original Message-
From: Colleen Forster via Histonet
Sent: Friday, May 17, 2024 3:07 PM
To: Jay Lundgren
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Agarose embedded tissue
Chistopher,
Were these small pieces of tissue? If they are in an agar (such as
histogel) they would need to have been processed overnight to ensure the
agar is completely dehydrated during processing. The sample, no matter how
small, is protected and processes perfectly. IF you ran a short run
Where did the agarose come from?
On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
>
> Hello everyone!
>
> I'm having trouble sectioning tissue array blocks where the array is in
> agarose embedded into a paraffin block. I've
Hello everyone!
I'm having trouble sectioning tissue array blocks where the array is in
agarose embedded into a paraffin block. I've chilled the blocks and I'm
sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge
blade. The paraffin surrounding the agarose sections
