You did not specify which enzyme you were using, but I am assuming
proteinase K. If an hour's retrieval is too much, and no retrieval is too
little, I would suggest running some (alcohol fixed) controls at 15, 30, and
45 minutes in the 95C, or 1,2,3, and 4 minutes in the enzyme. Evaluate the
alin step will cross link the proteins requiring
epitope retrieval.
René J.
--- On Tue, 2/2/10, Martin, Erin wrote:
From: Martin, Erin
Subject: [Histonet] Alcohol fixation and immuno
To: "histonet"
Date: Tuesday, February 2, 2010, 10:24 AM
Good morning everyone,
We occasionally receive sp
Exactly!
Your tissue suffer (that is the appropriate word) a double fixation,
probably partial in both, but the formalin step will cross link the proteins
requiring epitope retrieval.
René J.
--- On Tue, 2/2/10, Martin, Erin wrote:
From: Martin, Erin
Subject: [Histonet] Alcohol fixation
Good morning everyone,
We occasionally receive specimens that were fixed in alcohol but processed on
our machine, which starts in formalin. In our typical antigen retrieval we
either use a 95 degree waterbath for an hour or enzyme for 5 minutes. On
alcohol fixed tissues this chews up the tissu
