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Having issue with Millipore Alpha Dystroglycan antibody. Millipore recommend 1:100 dilution so we tried various titrations ranging from 1:100, 3:200 to 9:200, normal and abnormal muscle tissue with very poor results. After staining up to 9:200 dilution immuno come out patchy not strong enough Any one on the histonet list use this antibody, if so at what dilution. any input would be really appreciated Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
