This is a well known phenomenon of amyloid staining and it is fairly
common. It is due to the fact that amyloid is a very variable material
and different samples may react differently to the dyes. I suggest you
restain using Highman's congo red method
What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 1/23/15, 10:43 AM, Jeffrey Robinson
jrobin...@pathology-associates.com wrote:
Greetings to all
12:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Amyloid by Congo Red
Greetings to all Histotechs- Here's an amyloid question for the braintrust.
We are cutting our slides and controls at 9 and staining in Congo Red for 1
hour. The control stains fine but the patient tissue
Greetings to all Histotechs- Here's an amyloid question for the braintrust.
We are cutting our slides and controls at 9 and staining in Congo Red for 1
hour. The control stains fine but the patient tissue is staining negative even
on cases that the pathologist assures us should be positive
