: [Histonet] Annexin V IHC staining in mouse xenograft
Hi,
Have you considered running the antibody through some antibody
purification beads. It isn't really a common histolgy thing to do, but the
process isn't terribly difficult. It really sounds like a dirty antibody is
at fault. It wil
ncentrated
antibody. There are some commercially available kits to do this. I believe
Invitrogen carries one and I'm almost sure Chemicon does too.
Amos
Message: 1
Date: Tue, 11 Oct 2011 17:11:38 +
From:
Subject: [Histonet] Annexin V IHC staining in mouse xenograft
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Message-ID:
Content-T
Hi Aprill,
I am not sure if you are using an antibody against Annexin V or if you
are trying to stain with Annexin V (use it as a detection agent).
Annexin V is a protein that binds to phosphatidyl serine. It is often
used as a tool to detect early stages of apoptosis when the plasma
membrane "fl
s/dilutions
>that you use, someone on Histonet may be able to answer your question.
>
>Jan Shivers
>Univ. of Minnesota
>
>- Original Message -
>From:
>To:
>Sent: Tuesday, October 11, 2011 12:11 PM
>Subject: [Histonet] Annexin V IHC staining in mouse xenograft
&
] Annexin V IHC staining in mouse xenograft
Hi histonetters,
I need some help. I am running an antibody from Biosensis called Annexin V
in mouse xenograft brain samples with human glioma tumors treated and
non-treated. I have incredible background that I can not get rid of any of
my samples. I
Hi histonetters,
I need some help. I am running an antibody from Biosensis called Annexin V in
mouse xenograft brain samples with human glioma tumors treated and non-treated.
I have incredible background that I can not get rid of any of my samples. I
have used a protein block, rodent tissue b
Histonetters: Looking for Annexin V Ab porcine reactiveanybody out
there used Annexin V on piggies for histology? What is the scoop? I'd
rather not re-invent the wheel. I am sure, someone has done ths.CB
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