Hi Aprill, I am not sure if you are using an antibody against Annexin V or if you are trying to stain with Annexin V (use it as a detection agent).
Annexin V is a protein that binds to phosphatidyl serine. It is often used as a tool to detect early stages of apoptosis when the plasma membrane "flips" and exposes phosphatidyl serine on the outside of cells, whereas in normal live cells it would be on the inside. It is used eg. in flow cytometry on unfixed cells. Dead cells or permeabilized cells would be all positive as the reagent (Annexin V) would have access to the inside of cells and react with phosphatidyl serine in the inner leaflet of the membrane. I don't think it could work on tissues in paraffin sections. But somebody may prove me wrong... Sarka Lhotak McMaster University, Hamilton, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
