Hi All,
Has anyone had experience doing IHC on FFPE tissues with Collagen Type IV subunit antibodies? These are Collagen Type IV alpha 1, alpha 3 and alpha 5 chains. They are used for the diagnosis of Alport syndrome and all antibodies are from KAMIYA BIOMEDICAL COMPANY, Seatle. At present, it seems that only COL4A5 is working on FFPE tissues. I tried with (or without) various pre-treatment before the primary incubation with the dilution of 1:50 to 1:500. I am using BondMax autostainer and I also tried Decloaking chamber HIER with manual staining. I was wondering whether there would be other source for these antibodies. Thank you. Regards, Young Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kw...@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
