When doing an initial antibody validation CAP requires at least 90% concordance
between the new test and the comparator test or expected values.Are there
specified requirements for sensitivity, specificity and accuracy?
Thanks in advance for any help provided!
Martha Ward, Manager, Molecul
comment as to whether it might be usable
for a limited IH operation?
Thank you,
Matthew Fleming, MD
Fleming Dermatopathology
Milwaukee, WI
--
Message: 2
Date: Fri, 16 Mar 2018 06:54:30 -0700
From: "Paula"
To:
Subject: [Histonet] Antibody Validati
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody Validation CLIA
CAP suggests running 10 negative and 10 positive cases for non-prognostic
markers.
CAP required running 20 negative and 20 positive cases for prognostic markers.
In the event the case volume is less than the sugg
Hi Paula,
Let me first say I am Canadian and my lab is not governed by CLIA or CAP.
But for what it is worth, here are my thoughts...
When thinking about validating antibodies for IHC you must first consider
whether the antibody in question is Class-I (prognostic eg Breast markers,
CD117, etc) or
@lists.utsouthwestern.edu]
Sent: Friday, March 16, 2018 8:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody Validation CLIA
WARNING: This email appears to have originated outside of the UW Health email
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know
the
Hello,
We've been discussing about the quantity of slides to run as a validation
for IHC antibodies. We are governed by CLIA, and we would like to know if
there is a set number of slides to run for a particular antibody we would
like to bring in-house for Validation. I think CAP requires 20 sl
: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 16, 2017 7:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ANtibody validation records
Would anyone be willing to share how the document their new antibody
validations when adding a new
Would anyone be willing to share how the document their new antibody
validations when adding a new antibody test to the panel of tests your lab
performs. I currently have a basic packet but feel it could be better and
wanted to see how everyone else performs/records their testing. The people
who we
I am wondering what process labs are doing to validate new antibodies per CAP
guidelines?
Thanks!
Tanya
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net
Thi
Hi,
How are you guys doing? I hope you are doing great. Please I will appreciate
it, if you guys have a procedure on the ANTIBODY VALIDATION and would like to
share it with me.
Thanks
Wilson
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
n.edu
Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE
Hi,
How are you guys doing? I hope you are doing great. Please I will
appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and
would like to share it with me.
Thanks
W
Hi,
How are you guys doing? I hope you are doing great. Please I will
appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and
would like to share it with me.
Thanks
Wilson
___
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Histonet@lists.utsouthw
Buesa
Sent: Tue 6/15/2010 9:49 AM
To: histo...@pathology.swmed.edu; teri.hall...@midmichigan.org
Subject: Re: [Histonet] Antibody Validation
Teri:
You are right about the validations you propose although I am not surprised
that your vendor does not think it is necessary. They are in the busine
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Tuesday, June 15, 2010 9:01 AM
To: Rene J Buesa; histo...@pathology.swmed.edu; teri.hall...@midmichigan.org
Subject: RE: [Histonet] Antibody Validation - long response
Bottom line it&#
e 15, 2010 8:50 AM
To: histo...@pathology.swmed.edu; teri.hall...@midmichigan.org
Subject: Re: [Histonet] Antibody Validation
Teri:
You are right about the validations you propose although I am not surprised
that your vendor does not think it is necessary. They are in the business of
selling and
There are other issue that you have to take into consideration with both
instruments, With digital image analysis you also have to look at the
questions that CAP just instituted for this. This includes the machine
being run by someone that can do high complexity testing. This means
that you have
Subject
Re: [Histonet] Antibody Validation
06/15/2010 10:49
AM
sake.
There is where the difference resides. Ignore your vendor and keep validating
your protocols.
René J.
--- On Tue, 6/15/10, teri.hall...@midmichigan.org
wrote:
From: teri.hall...@midmichigan.org
Subject: [Histonet] Antibody Validation
To: histo...@pathology.swmed.edu
Date: Tuesday, June 15,
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validate
HI Everyone and Happy Easter!
Just wondering how you validate your antibodies?
I've read that some antibodies use negative controls with the genes or proteins
removed and that the positive controls have the protein added. Is that the
procedure for research or manufacturing situations?
Thanks,
Ca
.2758
Cell: 408.335.9994
(W) E-Mail: aallison-ta...@apmglab.com
(P) E-Mail: akemiat3...@yahoo.com
--- On Mon, 9/14/09, Cynthia Robinson wrote:
From: Cynthia Robinson
Subject: Re: [Histonet] antibody validation Thanks, but need Non-"U" also
To: "Akemi Allison-Tacha"
Date: Monday, Septem
...@apmglab.com
(P) E-Mail: akemiat3...@yahoo.com
--- On Fri, 9/11/09, Akemi Allison-Tacha wrote:
From: Akemi Allison-Tacha
Subject: [Histonet] antibody validation
To: "histonet"
Date: Friday, September 11, 2009, 8:20 AM
Good Morning and Happy Friday out there in Histo-Land!
I would
: Saturday, 12 September 2009 1:21 AM
To: histonet
Subject: [Histonet] antibody validation
Good Morning and Happy Friday out there in Histo-Land!
I would like your assistance in an issue that I have just become aware of
regarding antibody validation. I have been requested to bring on-board
ap
visor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: Friday, September 11, 2009 8:21 AM
To: histonet
Subject: [Histonet] ant
Good Morning and Happy Friday out there in Histo-Land!
I would like your assistance in an issue that I have just become aware of
regarding antibody validation. I have been requested to bring on-board
approximately (7) new antibodies in the immediate future, and several
antibodies later.
Altho
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