Cap states, "should test a minimum of 10 positive and 10 negative tissues".
Does not state "cases" but "tissues", so we used a block for positive and
negative from the same case.
Thanks,
Tim
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
ent: Friday, March 16, 2018 11:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody Validation CLIA
CAP suggests running 10 negative and 10 positive cases for non-prognostic
markers.
CAP required running 20 negative and 20 positive cases for prognostic markers.
In the eve
Hi Paula,
Let me first say I am Canadian and my lab is not governed by CLIA or CAP.
But for what it is worth, here are my thoughts...
When thinking about validating antibodies for IHC you must first consider
whether the antibody in question is Class-I (prognostic eg Breast markers,
CD117, etc) or
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Sent: Friday, March 16, 2018 8:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody Validation CLIA
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Hello,
We've been discussing about the quantity of slides to run as a validation
for IHC antibodies. We are governed by CLIA, and we would like to know if
there is a set number of slides to run for a particular antibody we would
like to bring in-house for Validation. I think CAP requires 20
