You can also perform BrdU on the MMA sections the BrdU label is quite hardy.
Robert Schoonhoven
From: Nancy W. Troiano
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, April 21, 2009 1:37:01 PM
Subject: [Histonet] BRDU, Tetracycline, calcein in bone
I
I would recommend fixing the bones in 70% ethanol, embedding in
methylmethacrylate undecalcified and cutting 8 - 10 micron thick sections
for visualization of tetracycline and/or calcein labels. Decalcification
will remove fluorescent labels bound to the mineral in bone as mineral is
removed
