Center
From: Bartczak, Joanna
Sent: Thursday, August 13, 2020 10:10 AM
To: Paula Keene Pierce ; Morken, Timothy
; Histonet
Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond
I have seen this phenomenon years ago on the Bond III when I worked at a
different laboratory. If I
Keene Pierce
Sent: Wednesday, August 12, 2020 6:42 PM
To: Morken, Timothy ; Histonet
Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond
I have seen this when using Sequenza cover plates.
My thoughts were that it is dissolved gases in the washes. As I noticed that
when filling
Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue
sections that cause unstained (ie, clear) areas where bubbles have formed?
We've been trying things like extra buffer wash before reagent, new slide trays
(tech rep suggestion). It is seen on single- antibody slides
