I am using Fix-All from Surgipath for bone marrow fixation. I empty the
syringe into the fixative as soon as it is handed to me (no messing around with
clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and
our normal processing with 10%NBF. The pathologist is very
Acetic Zinc Formalin
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Good afternoon everyone,
In past years our lab used B-5 fixative on bone marrow biopsies for better
immunostaining.
With the chemical hazards of B-5 we switched to AZF fixative and have been
using this for the past few years. I am just curious what everyone else is
using for fixative on their
Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is
perfect.
René J.
--- On Tue, 3/2/10, Amy Farnan farn...@nehealth.com wrote:
From: Amy Farnan farn...@nehealth.com
Subject: [Histonet] bone marrow fixative
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, March
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow fixative
Good afternoon everyone,
In past years our lab used B-5 fixative on bone marrow biopsies for better
immunostaining.
With the chemical hazards of B-5 we switched to AZF fixative and have been
using this for the past few