[Histonet] Bone Marrow Fixative

2010-03-04 Thread Kelly Larson
I am using Fix-All from Surgipath for bone marrow fixation. I empty the syringe into the fixative as soon as it is handed to me (no messing around with clots). I fix for 1-2 hours in Fix-All then transfer to a screen cassette and our normal processing with 10%NBF. The pathologist is very

Re: [Histonet] bone marrow fixative

2010-03-03 Thread Amy Farnan
Acetic Zinc Formalin Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. ___ Histonet mailing list

[Histonet] bone marrow fixative

2010-03-02 Thread Amy Farnan
Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their

Re: [Histonet] bone marrow fixative

2010-03-02 Thread Rene J Buesa
Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is perfect. René J. --- On Tue, 3/2/10, Amy Farnan farn...@nehealth.com wrote: From: Amy Farnan farn...@nehealth.com Subject: [Histonet] bone marrow fixative To: histonet@lists.utsouthwestern.edu Date: Tuesday, March

RE: [Histonet] bone marrow fixative

2010-03-02 Thread Rathborne, Toni
To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow fixative Good afternoon everyone, In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining. With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few