Tyrone - I have been working with fish (whole and pieces and parts) for years. The fixative of choice for whole fish as been Davidson's. It contains formalin, alcohol, acetic acid and water. After the whole fish is opened, the acetic acid begins to soften the harder tissues while the formalin fixes the rest. Since there is alcohol, the tissues are then placed in 70% alcohol and processed. The length of time for fixation depends on the size of the fish of course. You'll just have to find the times. Many of the small fish (fingerlings) have been ready in 24 hours. But many of the fish we get have been fixed for a week or more and results are really good. Contact me personally if you need more information. Cheryl
Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet