Patrick, or anyone else using the alpco product...can you give some details/feedback on how this anti human C4d (FITC-Conjugated) is working for you, and whether you were able to replace two steps with one for C4d IF staining? I checked that website, and the pdf on this product reads: "The antibody detects human complement split product C4d and has been tested for use on paraffin sections and frozen sections of human Tissue. " We've switched our goat anti-mouse currently used for C4d immunofluorescence, and would prefer to switch to a conjugated product. It sounds like something we'd like to try. Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada
Try the C4d directly labeled antibody from alpco www.alpco.com anti human C4d (FITC-Conjugated) Cat #: 04-BI-RC4D-FITC Good luck. On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta <rbut...@ameripath.com> wrote: > I'm having trouble with my C4d IF stain. I'm using AbDirect/Serotec, mouse > monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec > diluted 1:25 for ½ hour. This is not working and I've tried different > dilutions with no success. Can anyone share their protocol? > > Rosie Butler, > AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK > 73112/405.842.7575 - Office > 405.650.8921 - Cell/405.841.2002 - Fax -- >>Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
