Re: [Histonet] CD105 and CD166 on human bone/bone marrow

2008-10-14 Thread Greg Dobbin
Liz, If your controls are fine but your bone marrows are not, could it be the decalcification process that is damaging these antigens? Try using EDTA for decal instead of formic acid. Just a thought. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen

[Histonet] CD105 and CD166 on human bone/bone marrow

2008-10-14 Thread Liz Chlipala
Hello All I need a bit of help here. We have been trying to localize CD105 and CD166 in human bone. Our control tissue is staining just fine (human tonsil and human skin) but we have been unable to demonstrate any of these cells within the bone marrow. Any help would be appreciated, especially