logy Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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Message: 2
Date: Thu, 30 Jun 2016 16:57:53 -0500
From: "Croix, Denise" <denise.cr...@roche.com>
To: histonet@lists.utsouthwestern.edu
S
Hi Cathy,
When I was doing work with NHP tissue (both flow cytometry and IHC) there
were several sources that I utilized for info on antibodies that were
cross-reactivity with various NHP species. The NIH hosts a NHP reagent
resource website (nhpreagents.org) that shows which commercially
, 2016 8:07 AM
To: histonet@lists.utsouthwestern.edu
Cc: Eddie Martin
Subject: Re: [Histonet] CD34 for primate tissue
Rhesus is a species of old world monkey. There are a couple of other companies
that have this same clone and on their datasheets they say it cross reacts with
rhesus monkey
22 PM
> To: Cathy M. Mathis/Comparative Medicine
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] CD34 for primate tissue
>
> What species is the kidney you are trying to stain with IHC. Because you
> mentioned that you used normal human kidney and it worked , but
/Comparative Medicine
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD34 for primate tissue
What species is the kidney you are trying to stain with IHC. Because you
mentioned that you used normal human kidney and it worked , but Rhesus kidney
didn't stain. I'm wondering
s species?
> More suggestions please? Thank you all for your time.
> Cathy
>
> -Original Message-
> From: Eddie Martin [mailto:edmarti...@gmail.com]
> Sent: Wednesday, June 29, 2016 5:34 PM
> To: Cathy M. Mathis/Comparative Medicine
> Cc: histonet@lists.utsouthw
/Comparative Medicine
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD34 for primate tissue
Hi Cathy,
EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all
that is necessary. Please contact me if you need additional help.
Best,
Eddie Martin, HTL, QIHC
Hi Cathy,
EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all
that is necessary. Please contact me if you need additional help.
Best,
Eddie Martin, HTL, QIHC
edmarti...@gmail.com
Sent from my iPhone
> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative
Good morning my fellow Histo-netters,
Does anyone have experience with any CD34 antibody that would work in FFPE
rhesus tissues? A few companies have one with clone QEBend 10 and say that it
works but I have had no luck. I am using the Bond RX polymer system and I've
tried all the epitope
