I have running a experiment in rabbit that included the bone reconstruction
using mesenchymal stem cell seeded previously in calcium phosphate and calcium
silicate scaffold. I need to know if, after some time, the scaffold remains in
the tissue or they are reabsorbed and the bone is reconstruted. The technique
is parafin embeded tissues.
My question is: what kind of decalcification have I to used to
decalcificate the bone but that permit me to see the remained calcium phosphate
and calcium silicate scaffold?. Could the acid or EDTA eliminate completly the
remained scaffold?
Any opinion on your experience is appreciated.
Sincerely
Ana I. Nieto
Anapath Pathology Diagnosis
ani...@anapath.es
www.anapath.es
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