Hello, I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL 95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized water) and used the Hotchkiss-McManus PAS protocol to stain *Saprolegnia *fungi. The fungi are light green from the counterstain and not PAS-positive. I used fresh staining reagents and am wondering if the acetic acid in the Dietrich's fixative may have affected the cell walls. I'd appreciate your comments and suggestions.
Thanks very much! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
