Dear all, I have been looking for a protocol for staining connective tissue on semithin (0.5 um) araldite-embedded sections. I found at least two protocols mentioning double staining (methylene blue/basic fuchsin) but turns out that only their abstracts are available. Any experience with this? I am trying to determine whether connective tissue has invaded muscle territory or muscle degeneration is taking place. I know about Heidenheins's azan trichrome, but it requires paraffin embedding (does it?) and it would be better if I could do it on the araldite sections. Thanks to all, Daniel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
