tonet@lists.utsouthwestern.edu
Subject: [Histonet] Coverslipping Problem - Please Help!
Dear Histonet members,
I have been cryosectioning Xenopus laevis embryos this summer that have
undergone a whole mount chromogenic in situ. The embryos are then fixed
overnight in a formaldehyde solutio
Dear Histonet members,
I have been cryosectioning Xenopus laevis embryos this summer that have
undergone a whole mount chromogenic in situ. The embryos are then fixed
overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS
until they are cryoprotected in a sucrose so