RE: [Histonet] Coverslipping Problem - Please Help!

2010-07-30 Thread Collette, Nicole M.
tonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipping Problem - Please Help! Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solutio

[Histonet] Coverslipping Problem - Please Help!

2010-07-29 Thread Brian Rabe
Dear Histonet members, I have been cryosectioning Xenopus laevis embryos this summer that have undergone a whole mount chromogenic in situ. The embryos are then fixed overnight in a formaldehyde solution (1X MEMFA) at 4 C, then stored in 1 x PBS until they are cryoprotected in a sucrose so