I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting
is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting
at a lower temp and cutting thicker?
Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Good morning! My pathologists would like us to cut formalin fixed (not yet
processed) tonsil tissue on a cryostat for DIF staining. Has anyone done this?
I did a quick search that seemed to indicate that it was possible but that the
architecture of the cells would be altered because of ice cr
Hi,
We are searching for a fee for service lab experienced in cryostat sectioning
of animal and human tissues. Any recommendation is appreciated.
Thanks,
Shu-Cheng
Notice: This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse
Hi,
I have used a Leica cryostat for several years and found that for most
types of tissue, it cuts best at an angle set at 0. I am interested in
knowing how long it takes for the tissue to warm up from -80 to -20.
Danny Brawn
Prince Edward Island
Canada
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