Re: Re: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides

2009-04-19 Thread TF
are. Place the section directly on a warm (room temperature) slide and the section will adhere. Paula From: TF To: Histonet Sent: Friday, April 3, 2009 10:43:03 AM Subject: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides After we cut the brain sections on a cryostat

[Histonet] Cryostat sections mounting with 0.1M PB solution onto slides

2009-04-03 Thread TF
After we cut the brain sections on a cryostat (OCT embedded), we brush a drop of 0.1M PB on to the slide before mounting the sections. Do anyone know the side effect of this - for example, the sections will peel off during immunostaining? Another question is about the dry time after mounting and