Definitely do at least that one sucrose cryopreservation step you
mentioned. Even step up to it in 10% and 20% sucrose.
--On Wednesday, March 18, 2009 4:04 PM +1100 Adam Galle
adam.ga...@student.unsw.edu.au wrote:
Hi all,
Currently I am working with brains from 7 day old rat pups, that
Hi all,
Currently I am working with brains from 7 day old rat pups, that undergo an
hypoxic-ischemic injury (Levine/Vannucci technique). These brains are
unfixed, frozen in isopentane and cut at 20um on a croystat. These brains
are not cutting very well compared to an adult brain (potenially
