RE: [Histonet] Cytoplasmic staining with Ki67 in frozen sections

2011-01-27 Thread Liz Chlipala
7; Subject: [Histonet] Cytoplasmic staining with Ki67 in frozen sections HI All, I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However,

[Histonet] Cytoplasmic staining with Ki67 in frozen sections

2011-01-27 Thread James S.
HI All, I've just been trying out a Ki67 antibody from Abcam (rabbit polyclonal) on some frozen mouse tissues. Staining looked ok in the colon with nuclear localisation in the cells of the basement membrane. However, what I'm really interested in is the spleen and the staining here was a mess!