I do manual IHC-DAB regularly
DAB shouldn't "go off" after freezing unless you have somehow oxidised it
during mixing/aliquoting.
It would then show as a dark brown aliquot
I state this because I still make up my own DAB from dry powder ( have been
doing for > 20yrs)
SIgma D5637
I dissolve,
Hi everyone,
I am doing free-floating immunohistochemistry with brain slices.
That is only the context. The issue is that I always prepare my DAB
(Hydrochloride sigma powder) aliquot it and store it in -20°C. But
recently, once I thaw it the DAB quality decreases a lot, to almost not
have
Better try another counterstaining. Will be easier in the long run.René
On Monday, August 5, 2019, 01:08:38 PM EDT, Alonso Martínez Canabal via
Histonet wrote:
Good Afternoon.
It is great to be in this list again. My first e-mail is to ask
your advise about a little problem
Good Afternoon.
It is great to be in this list again. My first e-mail is to ask
your advise about a little problem that we have in the lab.
We are doing immunohistochemistry in brain tissue using DAB enhanced
with nickel ammonium sulfate. The result is great, with a very clean
Good morning,
I am currently experiencing an issue with DAB concentrating on certain bone
areas of my slide. I am working with decalcified—EDTA , FFPE- rabbit mandible
(some tissue attached). It is a new DAB kit from BioCare. It’s thoroughly mixed
(and new) before application in a humidity
Store it and give it to your Chemical people ( sensible) orfollow John
Kiernan's most excellent advice that he garnered ( in Histonet archives)
I wonder what people do, who use polymer DAB kits?
I reckon they chuck it down the sink.do the Suppliers of such kits advice
appropriate
Gathering data to make a decision on how labs neutralize and dispose of DAB
waste. Any input would be greatly appreciated.. Thanks..Cindy
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That's correct if you do not quench endogenous peroxidase activity completely
you would be able to visualize the RBC's but maybe in instances like this it
might be better to have the rbc's stain a different color than the DAB they
would be easier to recognize, especially if they are
Hi Histonet, Marcus and Liz
I agree with everything Liz says about precipitated DAB being wonderfully
robust so that you can do a whole range of stains successfully after using it.
I am slightly puzzled that Marcus wants to reveal RBCs after a DAB reaction.
Doesn't it just happen any way?
Hi Thomas,
I've see that Dako's DAB Away is composed of 1% Oxalic acid in water
instead other DAB Aways (like the one you are using) are composed of
sulfuric acid 10% in water.
Dario
2014-06-06 17:53 GMT+02:00 Thomas Pier t...@medicine.wisc.edu:
Hello Histonet,
We have been using Lab
Hello Histonet,
We have been using Lab Vision's DAB away to clean our Autostainer 360. However
it seems a little wasteful to buy something that we can't use up before it
expires. Do any of you have any exerpience with lower cost alternatives?
Thanks,
Tom
Good morning all,
I posted yesterday regarding the use of benzidine for the detection of
hemoglobin. My pathologist believes that standard DAB can be used instead of
benzidine solution but I can't find any references for using DAB as a
hemoglobin stain. Do any of you fine histo folks do
(ASCP) QIHC
From: mw...@wakehealth.edu
To: wben...@cua.md; davidclar...@comcast.net;
histonet@lists.utsouthwestern.edu
Date: Thu, 11 Jul 2013 18:52:36 +
CC:
Subject: [Histonet] DAB enhancer
Sorry but I cannot get my messages to go through to the Histonet so I am
piggybacking onto
I have been asked by a new pathologist to look into a DAB enhancer for use on
our Bond III instruments. Are many of you using this on the Bond, or any
other staining platform and what are the pros and cons of its use?
Thanks!
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Sorry but I cannot get my messages to go through to the Histonet so I am
piggybacking onto a previous message:
I have been asked by a new pathologist to look into a DAB enhancer for use on
our Bond III instruments. Are many of you using this on the Bond, or any
other staining platform and
I don't know why anyone would want to use a DAB enhancer for slides prepared on
Leica-Microsystems' Bond Max platforms. The resulting immunoreactivity is
exceptionally Robust in my experience when using their Bond Polymer Refine
Detection kit. Could this request be for a protein target
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lori Harris
Sent: Monday, July 01, 2013 5:45 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] DAB Testing
Hello All!
Can someone recommend a company that does DAB waste testing? I would like to
get our waste tested from
Hello All!
Can someone recommend a company that does DAB waste testing? I would like to
get our waste tested from our Ventana Ultra. Thanks.
Lori A. Harris, HT (ASCP)
541-768-6078
Histology Section Lead
GSRMC Pathology Lab
3600 NW Samaritan Drive
Corvallis, OR 97330
Hello All,
Can anyone recommend a DAB kit that is reliable and reasonable priced? Also,
can someone shovel my driveway?
Thanks!
Sandy
Sandra Cheasty
Histology Necropsy Supervisor
UW-Madison, Wisconsin
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#@*% prep kits.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Monday, August 20, 2012 2:11 PM
To: Dorothy Glass; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] DAB precipitate
I have been getting something on some slides, not all that looks like bacteria
but it is not. It looks like Dab precipitate. It looks like the Dab is breaking
down, but only noticeable on the stains for H. pylori. I wash my water bath,
and changes my blades. It not occurs all the time, but it
@lists.utsouthwestern.edu
Subject: [Histonet] DAB precipitate on H. pylori
I have been getting something on some slides, not all that looks like bacteria
but it is not. It looks like Dab precipitate. It looks like the Dab is breaking
down, but only noticeable on the stains for H. pylori. I wash my water bath
as usual and
you will see clear and crisp counterstain.
If hematoxyline will be to much, then you can
differentiate it in 0.25% HCl 5-10-15 dips, then wash
buffer.
Hope this help.
Maxim Peshkov,
Russia,
Taganrog.
From: Megan French megan.fre...@mcri.edu.au
Subject: [Histonet] DAB haematoxylin
Hi all,
I have been working through an immuno using DAB and counterstaining with
harris haematoxylin.
Protocol for counterstain:
5 min h20
1 dip haematoxylin
1 min h20
2 dips Blue in ammonia
1 min h20
Dehydrate, clear, mount
I am finding that my slides are coming out reay
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela
Bodemer
Sent: Tuesday, November 01, 2011 8:47 PM
To: Histonet@lists.utsouthwestern.edu
Cc: Susan Lapthorne
Subject: [Histonet] DAB Immuno and alcian blue counter staining
Hi all,
Is it possible to do an immuno with DAB detection
Hi all,
Is it possible to do an immuno with DAB detection and counterstain it
with alcian blue at the end to detect Goblets cells? I've tried it and
the DAB disappears under the staining.
Thanks,
Daniela
__
This email has been
Hi,
I need some information on how to block endogenous peroxidase effectively
in liver frozen sections, as I need to do DAB staining.
What blocking buffer will work best for blocking in liver frozen
sections.
I appreciate any information on this issue.
Thanks,
Reena
Hello Histonetters,
I am currently trying to work out a new DAB protocol to be used with
IHC on FFPE sections.
I am testing three different protocols, and am having limited success.
The first protocol requires me to make a DAB stock solution of 10mg in
1 ml dH20, which is then filtered and
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Thursday, 8 July 2010 7:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB
Hello Histonetters,
I am currently trying to work out a new DAB protocol to be used with IHC on
FFPE sections.
I am
I have this DAB staining protocol for IHC. It was used on the DAKO autostainer
and incubated for 5min. Our controls using the DAKO DAB chromogen kit worked
fine. Does anyone have any experience with a DAB protocol similar to this or
have any other information to add to this protocol? Is
.
--- On Thu, 9/17/09, Nancy Herman nancy.her...@inspection.gc.ca wrote:
From: Nancy Herman nancy.her...@inspection.gc.ca
Subject: [Histonet] DAB Chromogen
To: histonet@lists.utsouthwestern.edu
Date: Thursday, September 17, 2009, 10:12 AM
I have this DAB staining protocol for IHC. It was used
Hi all,
I have been having a problem with counterstaining for nuclei - I'm
looking at mouse lung tissues and staining macrophages with F4/80 (rat
anti-mouse). I use a negative serum control and a rat IgG isotype
control. I see very clear staining of macrophages - but when I
counterstain with
Hi Dr. Kumar. We use a 0.05M Tris buffer pH 7.7-7.9. We add 3% H2O2 right
before use. We use our own stock DAB. Perhaps your DAB was too dilute? I
hope this info helps. If you would like more specifics, you can contact me.
Patti Loykasek BS, HTL, QIHC
Clinical Lab Supervisor
PhenoPath
hi every one,
I am using Labvision HRP polymer secondary antibody with
DAB chromogen, funny thing is that i have exhausted the DAB substrate buffer.
so i tried with PBS 7.4 and h2o2 and chromogen and i found out that nothing
worked. can anyone tell me a protocol or recipe
Hello Jennifer,
We are a hospital site and operate 2 Dako Autostainers. We collect our DAB
waste into empty plastic carboys from Coulter Diluent used in our Hematology
Department. These containers are encased within a cardboard cover. They are
very convenient as we can place the hose directly
Greetings
I recall that years ago Biomeda had a product called Stable DAB. It was
a ready-to-use product that was stored in the freezer. Does anyone know
if it is still being produced and whom I should contact?
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10
...@catholichealth.net
To: histonet@lists.utsouthwestern.edu
Sent: Tue, 17 Mar 2009 8:36 am
Subject: [Histonet] DAB
Greetings
I recall that years ago Biomeda had a product called Stable DAB. It was
a ready-to-use product that was stored in the freezer. Does anyone know
if it is still being produced and whom I
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