as usual and
you will see clear and crisp counterstain.
If hematoxyline will be to much, then you can
differentiate it in 0.25% HCl 5-10-15 dips, then wash
buffer.
Hope this help.
Maxim Peshkov,
Russia,
Taganrog.
> From: "Megan French"
> Subject: [Histonet] DAB haematoxylin cou
Hi all,
I have been working through an immuno using DAB and counterstaining with
harris haematoxylin.
Protocol for counterstain:
5 min h20
1 dip haematoxylin
1 min h20
2 dips Blue in ammonia
1 min h20
Dehydrate, clear, mount
I am finding that my slides are coming out reay
