Someone sent me a question regarding DAPI staining ( I generally use Hoechst
)...but anyway, they are having smearing of nuclear contents. I would guess
that is from rupturing of the nuclear membraneover fixation? Over air
drying? They are fixing 10 mins in cold acetone and 10 mins to air
Hi Carol,
Does this person put them in a freezer for 20 mins or so after DAPI
staining? Someone once told me this helps with smearing. I'm not really
sure if its true, but couldn't hurt to try it.
Mark
On Thursday, August 30, 2012, Barone, Carol wrote:
Someone sent me a question regarding
Hi everyone,
I am in a rush to finish my master's thesis, but I needed to know
exactly if it was possible to stain methacrylate embedded sections
with DAPI and if so does anyone have a method for this? I am currently
looking at cell distribution within these scaffold constructs that I
have
